Researchers presents a novel less destructive and relatively straightforward protocol that reduces damage, increases temporal resolution, and improves the accuracy of age-alignment between individuals.
It is based on the sampling of small (1 mm diameter) cylindrical plug transects from a thin section, along with an age-alignment scheme predicated on average growth rates for dentine areas. The proposed protocol is readily applicable and more anatomically sensitive than horizontal slicing. Micro-samples are smaller (in both length and depth), hence minimizing temporal overlap, and avoid directions that may contravene growth pattern. They completely circumvent areas where secondary and tertiary dentine or cementum can be deposited. Age-alignment is improved by using growth ratios of anatomical tooth areas. This method minimizes destruction, enables finer temporal resolution and facilitates data comparison.
Voir en ligne : A guide for an anatomically sensitive dentine microsampling and age‐alignment approach for human teeth isotopic sequences